Sample, Transfer, and Blocking Problems Identified In our previous videos, we addressed potential problems in samples, transfer conditions, and blocking methods. These issues might result in a lack of signal or faint bands. Transfer Conditions Issue Membrane Treatment Problem Blocking Issues
Struggling with weak or absent bands in your Western blot experiments? 🧪 Watch this detailed guide to troubleshoot and overcome common challenges faced during Western blotting. From optimizing transfer conditions to ensuring proper antibody selection, this video provides step-by-step solutions to enhance band visibility and accuracy in your protein analysis. Join us as we delve … Read more
e-BLOT brought the Touch Imager, which has won numerous awards in China, to the World’s Leading Trade for laboratory technology, analysis, biotechnology and analytica conference in Germany. Details as below: Date: June 21-24 2022 Address:Messe München exhibition center A3 Hall 513B Booth Quick overview of product features Two orders of magnitude higher than cooled CCD on sensitivityTwo … Read more
1.Preparing the sample: -Cutting 20 um frozen sections to a slide, 3-5 of them; depends on the tissue size. (The slides can store at –80C for later study) -Air dry for 5 min or so. -Dissolution: Triple-detergent lysis buffer (from Sambrook Fritsch Maniatis): 50 mM Tris-HCL 150 mM NACL 0.1% SDS 100ug/ml PMSF 1ug/ml Aprorinin … Read more
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