Specialized Western Blot Imaging System
More Applications:
- Southern Blot
- Northern Blot
- Dot Blot
- Isotope Imaging
Western blot as a classical protein quantification technique has been preceived as the golden standard for protein expression studies and has become an essential experiment for every molecular biology laboratory. However, the development of western blot technology has been very slow, among which the imaging detection technology has suffered from stagnant development for over 20 years.
The earliest imaging method adopted was DAB colorimetry, which had been eliminated because of its lack of sensitivity and quantitative range.
The chemiluminescence method was later emerged. There are 2 imaging methods used- darkroom filming and cold CCD imaging.
- The darkroom filming method features a very limited quantitative range that makes accurate quantitation even impossible. Therefore it is gradually abandoned by scientists.
- Nowadays the cold CCD imaging remains widely-used. The method replying on lens to fulfil the imaging process makes the imaging distance too long, plus the small-sized chip, thus resulting in even lower imaging sensitivity compared to darkroom filming. Its quantitative range is also too narrow to meet the increasing requirements of scientific researches.
Some people also try to use fluorescence imaging to do western imaging. Yet due to the fluorescence background interference of the film, fluorescence methods are far less sensitive than chemiluminescence methods, so they are also rarely used.
The revolutionary touch chemiluminescence imaging technology of e-Blot makes full advantage of the high sensitivity of the chemiluminescence method, overcomes many shortcomings of cold CDD imaging, and improves the imaging sensitivity, 2-3 logs higher quantitative range and efficiency, leading western imaging into a whole new era.
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New technology allows you to design the experiment with confidence
Two orders of magnitude higher than cooled CCD on sensitivity
While most x-ray film and cooled CCD western blot imaging systems may skip on weaker signals on a given samples, the e-Blot Touch Imager’s sensitive imaging chips bring them all out.
Adjustable exposure level allows for stronger bands to be regulated while weaker signals are encouraged to appear for accurate data acquisition. The enhanced sensitivity also eliminates false findings that could consume more time for samples to be repeated and images recaptured.
Wide quantitative range, strong and weak signals are clearly visible
Ultra-sensitive imaging chips come with enhanced sensors that pick up even the faintest of signals in a sample that traditional x-ray film and CCD might miss and capture them all in a single image.
Signal variation is properly displayed on the image, helping scientists retrieve valuable data from even the rarest and low-abundant protein bands that will push forward research performed on the subject.
Two orders of magnitude higher than cooled CCD on quantitative range
As image capture is conducted through direct contact rather than distance capture, the dynamic range of the Touch Imager is proportionally higher than modern cooled CCD technology that the market offers.
The extended dynamic range also ensures that all details needed from the sample is recorded accurately, displaying various bends with precision accuracy.
Obtain Results Faster
95% experiments done within seconds
Thanks to the increased sensitivity and range of our Touch Imager’s photosensitive chip, 95% of the sample is captured in just 1 second.
The captured image of the sample provides accurate results based on specified details, eliminating the need to redo the experiment and significantly cutting time consumption by a great margin. Image editing and analysis are also available in the instrument for detailed images that help in better research.
Easy-To-Use By Everyone in The Lab
New technology does not need to be difficult to use, as our Touch Imagers feature a streamlined image capturing process that users can easily adapt to.
Simply load the sample into the device, press the designated button, and the image is captured into the system and ready for analysis. We made the process easy so anyone can use the Touch Imager and expect consistent results every time.
- Load the sample
- Press the button
- Get the result
E-blot Touch Viewer: All-in-One Software Automating Your Result Documentation
Have all the software and imaging applications on one handy instrument ensures that the needed images are captured for optimal data acquisition.
Image Acquisition: Capture Your Image Right Away
The built-in software makes it easy to take the imaging procedure by controlling the high-accuracy photosensitive chips utilized for the revolutionary contact imaging.
The smart interface provides an image preview to aid in image capture, with features like auto-focus and saturation to capture the right proteins.
Image Management: View and Keep Images Organized
The intuitive user interface allows direct access to images for reviewing, transfer, and sorting. View images immediately after capture, with a visible paper trail for proper documentation.
Export or share images using network connections or external drives, allowing for easy transfer of shared data for faster analysis and research.
Image Editing: Tailor the Images Your Way
Freely edit the captured images to highlight the data required for meetings, presentations, posters, and conferences with our built-in editing feature.
The easy-to-use software can adjust images, enhance and magnify image resolution you need, and combine images for better and more detailed data acquisition.
* Rotate *Zoom in *Zoom out *Crop *Flip
Image Analysis: Boost Your Analyzing Process
Perform on-the-spot or comparative analysis on your captured images using our reliable and accurate analytics feature. After capturing the image, you can easily get the analysis result through either automatic or manual analysis.
The Image Analysis feature streamlines the analytical process by handling validation tests, statistical review, and comparative analysis studies all within the system, reducing possible variations and errors in the process.
Capture mode | Auto/Manual |
---|---|
Full well capacity | 1,250,000.00 electrons |
Data transferring speed | 1000Mbps |
Light Sources Control | Chemoluminescence、Epi-white |
Start up waiting | No |
Exposure time | > 95% Samples are imaged within 1sec |
Photo sensor chip | 158cm2 |
User account management | Multi-user management |
Net weight | 4.35 KG |
Dimension (L x W x H) | 27cm×20.6cm×5.4cm |
Power supply | 100–250 V |
Operating temperature | 4–30°C |
Operating humidity | 10–85% relative humidity (noncondensing) |
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A:Touch Imager imaging is exactly the same as other chemiluminescent imaging methods. You only need to bring the finished WB membrane to the Touch Imager for imaging.
Touch Imager is suitable for imaging a variety of extremely weak autofluorescence signals, such as
Western blot chemiluminescence imaging
Southern blot nucleic acid blotting chemiluminescence imaging
Northern blot nucleic acid blot chemiluminescence imaging
Dot blot chemiluminescence imaging
Isotope labeling imaging
etc. ……
The Touch Imager is suitable for all types of WB chemiluminescence imaging, especially for weak signals and samples with large expression differences, such as: WB imaging of phosphorylated proteins, WB imaging of immunoprecipitation, WB validation of transgenic plants and transgenic animals, WB imaging of rare samples, etc.
A:It is ready to use without waiting.
A:There is no lens, the sample and the sensor chip are in direct contact, no need to adjust the focus
A:The software has various photo modes for all sample types.
Manual photo: Directly select the exposure time.
Automatic photo: signal accumulation mode, 30S continuous out of 8 different time exposure results.
Customized photography: independent setting of the first image exposure time and the accumulation time of different picture exposure.
Extreme HD mode: for taking pictures with very weak signals
A:Yes
A:No, the Touch Imager has undergone rigorous waterproof testing to ensure that water will never leak during use.
A:Use the wipe cloth to dry the residual luminous fluid, no other operation is needed.
A:Wipe the surface of the sample table with a cloth dipped in distilled water, dry it, cover the top of the instrument and cut off the power. The instrument should be stored in a cool, ventilated and dry environment.
A: The software comes with analysis and image editing functions such as gray value analysis, shear, rotation, etc. The software can be installed on your own computer. The software can be installed on your own computer, as long as the computer meets the software installation configuration requirements.
Make Your Western Blot Imaging Easier
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